CTFFIND3 and CTFTILT are two programs for finding CTFs of electron micrographs . The program CTFFIND3 is an updated version of the program CTFFIND2. Faster than CTFFIND 3 (x, depending on runtime parameters); Backward compatibility with CTFFIND 3 by giving --old-school-input. Should not break. Hi, I noticed some rather unusual results after running ctffind4 v on images previously processed by ctffind3. ctffind4 consistently gives.
Several programs are available for download: cisTEM: Fast and user-friendly single-particle image processing ; ctffind & ctftilt: CTF estimation ; Frealign. Estimating defocus and astigmatism of micrographs with CTFFIND3 script, and uses the shell script as input to the MRC program nudebeachbabes.info Of those, CTFFIND3 (Mindell and. Grigorieff, ) is widely used and thought to perform well under a range of circumstances (Marabini et al.
Hi Mike, I try to run ctffind3 with your suggested location. It shows some error: Estimating CTF parameters using Niko Grigorieff's CTFFIND3. We present two computer programs, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and. Hi Jenny, Yes, this is because relion still uses the older ctffind3, which did not ask for the amount of astigmatism. The release (expected.